Transcriptional networks in podocytes

We are aiming to decipher this network employing next-generation and single-nucleus sequencing techniques targeted to characterize transcriptomes (snRNAseq), transcription factor and chromatin dependent cistromes (ChIPseq, ATACseq, Cut&Tag), chromatin interactions (HiChIP), and spatial relationships (spatial transcriptomics) in both healthy and diseased podocytes in vivo. In particular, we are interested in transcriptional and epigenetic signatures of podocyte disease progression as well as spatial relationships between podocytes showing different damage states in murine models and human disease. In close collaboration with the Beyer lab in CECAD, we utilize innovative bioinformatic analysis approaches to determine trigger-specific and universal signaling pathways relevant to podocyte damage, which are then characterized in detail on the molecular level.

SELECTED PUBLICATIONS

Karaiskos, N., Rahmatollahi, M., Boltengagen, A., Liu, H., Hoehne, M., Rinschen, M., Schermer, B., Benzing, T., Rajewsky, N., Kocks, C., Kann, M., and Muller, R.U. (2018) A Single-Cell Transcriptome Atlas of the Mouse Glomerulus. J Am Soc Nephrol, 29(8): 2060-2068.

Maass, P.G., Aydin, A., Luft, F.C., Schachterle, C., Weise, A., Stricker, S., Lindschau, C., Vaegler, M., Qadri, F., Toka, H.R., Schulz, H., Krawitz, P.M., Parkhomchuk, D., Hecht, J., Hollfinger, I., Wefeld-Neuenfeld, Y., Bartels-Klein, E., Muhl, A., Kann, M., Schuster, H., Chitayat, D., Bialer, M.G., Wienker, T.F., Ott, J., Rittscher, K., Liehr, T., Jordan, J., Plessis, G., Tank, J., Mai, K., Naraghi, R., Hodge, R., Hopp, M., Hattenbach, L.O., Busjahn, A., Rauch, A., Vandeput, F., Gong, M., Ruschendorf, F., Hubner, N., Haller, H., Mundlos, S., Bilginturan, N., Movsesian, M.A., Klussmann, E., Toka, O., and Bahring, S. (2015) PDE3A mutations cause autosomal dominant hypertension with brachydactyly. Nat Genet, 47(6): 647-53.

Kann, M., Ettou, S., Jung, Y.L., Lenz, M.O., Taglienti, M.E., Park, P.J., Schermer, B., Benzing, T., and Kreidberg, J.A. (2015) Genome-Wide Analysis of Wilms' Tumor 1-Controlled Gene Expression in Podocytes Reveals Key Regulatory Mechanisms. J Am Soc Nephrol, 26(9): 2097-104.

Kann, M., Bae, E., Lenz, M.O., Li, L., Trannguyen, B., Schumacher, V.A., Taglienti, M.E., Bordeianou, L., Hartwig, S., Rinschen, M.M., Schermer, B., Benzing, T., Fan, C.M., and Kreidberg, J.A. (2015) WT1 targets Gas1 to maintain nephron progenitor cells by modulating FGF signals. Development, 142(7): 1254-66.

Desantis, A., Bruno, T., Catena, V., De Nicola, F., Goeman, F., Iezzi, S., Sorino, C., Gentileschi, M.P., Germoni, S., Monteleone, V., Pellegrino, M., Kann, M., De Meo, P.D., Pallocca, M., Hopker, K., Moretti, F., Mattei, E., Reinhardt, H.C., Floridi, A., Passananti, C., Benzing, T., Blandino, G., and Fanciulli, M. (2015) Che-1 modulates the decision between cell cycle arrest and apoptosis by its binding to p53. Cell Death Dis, 6: e1764.

Huang, J., Arsenault, M., Kann, M., Lopez-Mendez, C., Saleh, M., Wadowska, D., Taglienti, M., Ho, J., Miao, Y., Sims, D., Spears, J., Lopez, A., Wright, G., and Hartwig, S. (2013) The transcription factor Sry-related HMG box-4 (SOX4) is required for normal renal development in vivo. Dev Dyn, 242(6): 790-9.