We are aiming to decipher this network employing next-generation sequencing techniques targeted to characterize transcriptomes (RNAseq), transcription factor and chromatin dependent cistromes (ChIPseq), and chromatin interactions (HiC) in both healthy and diseased podocytes in vivo. As the actions of key signaling pathways activated in diseased podocytes such as Notch and Tgf-beta as well as some therapeutic agents such as corticosteroids converge within this podocyte nuclear network, its detailed characterization will yield important insight into podocyte biology in health and disease.
PD Dr. med. Martin Kann